Article
Flow cytometric measurement of functional and phenotypic P-glycoprotein.
Methods in molecular medicine - 1 Jan 2005
Pallis Monica, Das-Gupta Emma
Abstract excerpt
The measurement of functional and phenotypic P-glycoprotein by flow cytometry is suitable for cells in suspension, and is particularly appropriate for blood and bone marrow cells. We describe a functional assay for P-glycoprotein using rhodamine 123, an assay for daunorubicin accumulation, and an assay to measure P-glycoprotein levels using the MRK16 antibody. Our protocols include the use of an anti-CD45...
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