Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence.

doi:10.1016/j.str.2005.02.018

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Article

Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence.

Structure (London, England : 1993) - 1 May 2005

Townson Sharon A, Samuelson James C, Xu Shuang-Yong, Aggarwal Aneel K

Abstract excerpt

The type II restriction endonuclease BstYI recognizes the degenerate sequence 5'-RGATCY-3' (where R = A/G and Y = C/T), which overlaps with both BamHI (GGATCC) and BglII (AGATCT), and thus raises the question of whether BstYI DNA recognition will be more BamHI-like or BglII-like. We present here the structure of BstYI bound to a cognate DNA sequence (AGATCT). We find the complex to be more BglII-like with...

Topics

Bacterial Proteins
Base Sequence
Binding Sites
Crystallization
DNA
Deoxyribonucleases, Type II Site-Specific
Mutation
Nucleic Acid Conformation
Protein Conformation
Substrate Specificity

Source

Open in PubMed

Identifiers

PMID: 15893669
DOI: 10.1016/j.str.2005.02.018

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