Article
A double mutation in the extracellular Ca2+-sensing receptor's venus flytrap domain that selectively disables L-amino acid sensing.
The Journal of biological chemistry - 12 Aug 2005
Mun Hee-Chang, Culverston Emma L, Franks Alison H, Collyer Charles A, Clifton-Bligh Roderick J, Conigrave Arthur D
Abstract excerpt
The extracellular Ca(2+)-sensing receptor is activated allosterically by l-amino acids, and recent molecular analysis indicates that amino acids are likely to bind in the receptor's Venus flytrap domain. In the current study we set out to identify residues in the VFT domain that specifically support amino acid binding and/or amino acid-dependent receptor activation. Herein we describe two mutations of the...
Topics
- Allosteric Site
- Amino Acids
- Aminoacylation
- Blotting, Western
- Calcium
- Cell Line
- Cytoplasm
- DNA Mutational Analysis
- Humans
- Kinetics
- Mutagenesis, Site-Directed