Article
Outer pore topology of the ECaC-TRPV5 channel by cysteine scan mutagenesis.
The Journal of biological chemistry - 20 Feb 2004
Dodier Yolaine, Banderali Umberto, Klein Hélène, Topalak Ozlem, Dafi Omar, Simoes Manuel, Bernatchez Gérald, Sauvé Rémy, Parent Lucie
Abstract excerpt
The substituted cysteine accessibility method (SCAM) was used to map the external vestibule and the pore region of the ECaC-TRPV5 calcium-selective channel. Cysteine residues were introduced at 44 positions from the end of S5 (Glu515) to the beginning of S6 (Ala560). Covalent modification by positively charged MTSET applied from the external medium significantly inhibited whole cell currents at 15/44 positions....
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