Article
Identification of an L-phenylalanine binding site enhancing the cooperative responses of the calcium-sensing receptor to calcium.
The Journal of biological chemistry - 21 Feb 2014
Zhang Chen, Huang Yun, Jiang Yusheng, Mulpuri Nagaraju, Wei Ling, Hamelberg Donald, Brown Edward M, Yang Jenny J
Abstract excerpt
Functional positive cooperative activation of the extracellular calcium ([Ca(2+)]o)-sensing receptor (CaSR), a member of the family C G protein-coupled receptors, by [Ca(2+)]o or amino acids elicits intracellular Ca(2+) ([Ca(2+)]i) oscillations. Here, we report the central role of predicted Ca(2+)-binding site 1 within the hinge region of the extracellular domain (ECD) of CaSR and its interaction with other...
Topics
- Amino Acid Sequence
- Binding Sites
- Calcium
- HEK293 Cells
- Humans
- Intracellular Space
- Molecular Dynamics Simulation
- Molecular Sequence Data
- Mutant Proteins
- Mutation
- Phenylalanine
- Principal Component Analysis
- Protein Structure, Tertiary