Article
Lentiviral transduction of human hematopoietic cells by HIV‐1‐ and SIV‐based vectors containing a bicistronic cassette driven by various internal promoters
9 May 2005
Abstract excerpt
BACKGROUND: Lentiviral gene transfer into hematopoietic cells has been mostly optimized with vectors carrying a single reporter gene. For many clinical applications, lentiviral vectors should contain more than one gene because transduced cells should be enriched by a selectable marker or killed for safety reasons after use. Thus, we compared various vectors containing a bicistronic cassette driven by different...