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Article

Lentiviral vectors harboring a dual-gene system allow high and homogeneous transgene expression in selected polyclonal human embryonic stem cells.

Molecular therapy : the journal of the American Society of Gene Therapy - 1 Aug 2006

Ben-Dor Israel, Itsykson Pavel, Goldenberg Daniel, Galun Eithan, Reubinoff Benjamin E

Abstract excerpt

Genetic modification of human embryonic stem cells (hESCs) is highly valuable for their exploitation in basic science and therapeutic applications. Here we developed lentiviral vectors (LVs) constitutively expressing a reporter and a selectable marker to enable high and homogeneous transgene expression within polyclonal hESCs. LVs carrying GFP and a downstream puromycin resistance gene, linked by the...

Topics

Animals
Cell Line
Cell Line, Tumor
CpG Islands
Cricetinae
Drug Resistance
Embryo, Mammalian
Encephalomyocarditis virus
Genetic Vectors
Green Fluorescent Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Lentivirus

Phenotype

Promoter Regions, Genetic

Puromycin

Stem Cells

Transduction, Genetic

Transgenes

Source

Identifiers

PMID: 16632408
DOI: 10.1016/j.ymthe.2006.02.010

Genes

PGK1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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