Article
DNA sequence analysis of methylene chloride-induced HPRT mutations in Chinese hamster ovary cells: comparison with the mutation spectrum obtained for 1,2-dibromoethane and formaldehyde.
Mutagenesis - 1 May 1996
Graves R J, Trueman P, Jones S, Green T
Abstract excerpt
Glutathione-S-transferase-mediated metabolism of methylene chloride (MC) generates S-chloromethylglutathione, which has the potential to react with DNA, and formaldehyde, which is a known mutagen. MC-induced mutations in the HPRT gene of Chinese hamster ovary cells have been sequenced and compared with the mutations induced by 1, 2-dibromoethane (1,2-DEB), which is known to act through a glutathione conjugate,...
Topics
- Animals
- Base Sequence
- CHO Cells
- Cricetinae
- Cytosol
- Ethylene Dibromide
- Formaldehyde
- Hypoxanthine Phosphoribosyltransferase
- Liver
- Methylene Chloride
- Mice