Article
Importance of N-glycosylation positioning for cell-surface expression, targeting, affinity and quality control of the human AT1 receptor.
The Biochemical journal - 15 Aug 2005
Lanctot Pascal M, Leclerc Patrice C, Clément Martin, Auger-Messier Mannix, Escher Emanuel, Leduc Richard, Guillemette Gaétan
Abstract excerpt
GPCRs (G-protein-coupled receptors) are preferentially N-glycosylated on ECL2 (extracellular loop 2). We previously showed that N-glycosylation of ECL2 was crucial for cell-surface expression of the hAT1 receptor (human angiotensin II receptor subtype 1). Here, we ask whether positioning of the N-glycosylation sites within the various ECLs of the receptor is a vital determinant in the functional expression of...
Topics
- Animals
- Binding Sites
- COS Cells
- Calcium
- Chlorocebus aethiops
- Gene Expression
- Glycosylation
- Humans
- Kinetics
- Mutation
- Protein Binding
- Protein Conformation
- Protein Denaturation
- Protein Folding