Article
Different glycosylation requirements for the synthesis of enzymatically active angiotensin-converting enzyme in mammalian cells and yeast.
The Journal of biological chemistry - 15 Mar 1996
Sadhukhan R, Sen I
Abstract excerpt
For facilitating crystallization and structural studies of the testicular isozyme of angiotensin-converting enzyme (ACE,), we attempted the production of enzymatically active ACET proteins which are unglycosylated or underglycosylated. Expression in Escherichia coli of the rabbit ACET cDNA result...
Topics
- Animals
- Base Sequence
- Binding Sites
- DNA Primers
- DNA, Complementary
- Glycosylation
- HeLa Cells
- Humans
- Isoenzymes
- Male
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Peptidyl-Dipeptidase A
- Pichia
- Rabbits
- Saccharomyces cerevisiae
- Testis