Article
The zinc ion in the HNH motif of the endonuclease domain of colicin E7 is not required for DNA binding but is essential for DNA hydrolysis.
Nucleic acids research - 1 Apr 2002
Ku Wen-Yen, Liu Yu-Wen, Hsu Ya-Chein, Liao Chen-Chung, Liang Po-Huang, Yuan Hanna S, Chak Kin-Fu
Abstract excerpt
The HNH motif was originally identified in the subfamily of HNH homing endonucleases, which initiate the process of the insertion of mobile genetic elements into specific sites. Several bacteria toxins, including colicin E7 (ColE7), also contain the 30 amino acid HNH motif in their nuclease domains. In this work, we found that the nuclease domain of ColE7 (nuclease-ColE7) purified from Escherichia coli contains a...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Binding Sites
- Cations, Divalent
- Colicins
- DNA
- Deoxyribonucleases
- Dose-Response Relationship, Drug
- Endonucleases
- Escherichia coli
- Mutation
- Protein Binding
- Protein Structure, Tertiary