Article
Escherichia coli BTC, a human cytochrome P450 competent tester strain with a high sensitivity towards alkylating agents: involvement of alkyltransferases in the repair of DNA damage induced by aromatic amines.
Mutagenesis - 1 May 2005
Duarte Maria Paula, Palma Bernardo Brito, Laires António, Oliveira José Santos, Rueff José, Kranendonk Michel
Abstract excerpt
We report here on strain BTC, a new Escherichia coli mutagenicity tester strain for the expression of human cytochrome P450 (CYP) with an enhanced sensitivity for the detection of alkylating agents. This strain was developed first through knocking out of the genes ada and ogt in our previously developed strain BMX100, resulting in PD1000. Strain PD1000 demonstrated a significantly higher detection sensitivity...
Topics
- Alkyl and Aryl Transferases
- Alkylating Agents
- Amines
- Animals
- Aryl Hydrocarbon Hydroxylases
- DNA
- DNA Damage
- DNA Repair
- Escherichia coli
- Escherichia coli Proteins
- Humans
- Liver
- Methyltransferases