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Article

Determinants of the inherent strength of human 5' splice sites.

RNA (New York, N.Y.) - 1 May 2005

Roca Xavier, Sachidanandam Ravi, Krainer Adrian R

Abstract excerpt

We previously showed that the authentic 5' splice site (5'ss) of the first exon in the human beta-globin gene is intrinsically stronger than a cryptic 5'ss located 16 nucleotides upstream. Here we examined by mutational analysis the contribution of individual 5'ss nucleotides to discrimination between these two 5'ss. Based on the in vitro splicing efficiencies of a panel of 26 wild-type and mutant substrates in...

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Identifiers

PMID: 15840817
DOI: 10.1261/rna.2040605
PMCID: PMC1370755

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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