Article
A Pyrosequencing assay for rapid recognition of SNPs in Mycobacterium tuberculosis embB306 region.
Journal of microbiological methods - 1 Jul 2005
Isola Daniela, Pardini Manuela, Varaine Francis, Niemann Stefan, Rüsch-Gerdes Sabine, Fattorini Lanfranco, Orefici Graziella, Meacci Francesca, Trappetti Claudia, Rinaldo Oggioni Marco, Orrù Germano
Abstract excerpt
Ethambutol (EMB) is in use worldwide as a first-line anti-tuberculosis drug and substitutions in codon 306 of the embB gene are the most common mutations found in EMB resistant Mycobacterium tuberculosis (MTB) strains. Pyrosequencing is a real time sequencing method able to rapidly detect mutations in a large number of samples. Using this technique we analyzed, in parallel with conventional sequencing, a 24 bp...
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