Article
Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy.
The FEBS journal - 1 Apr 2005
Schleicher Erik, Hessling Benedikt, Illarionova Viktoria, Bacher Adelbert, Weber Stefan, Richter Gerald, Gerwert Klaus
Abstract excerpt
Cyclobutane-type pyrimidine dimers generated by ultraviolet irradiation of DNA can be cleaved by DNA photolyase. The enzyme-catalysed reaction is believed to be initiated by the light-induced transfer of an electron from the anionic FADH- chromophore of the enzyme to the pyrimidine dimer. In this contribution, first infrared experiments using a novel E109A mutant of Escherichia coli DNA photolyase, which is...
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