Article
Active site of Escherichia coli DNA photolyase: mutations at Trp277 alter the selectivity of the enzyme without affecting the quantum yield of photorepair.
Biochemistry - 19 Jun 1990
Li Y F, Sancar A
Abstract excerpt
Escherichia coli DNA photolyase repairs pyrimidine dimers by a photoinduced electron-transfer reaction. The enzyme binds to UV-damaged DNA independent of light (the dark reaction) and upon absorbing a 300-500-nm photon breaks the cyclobutane ring of the dimer (the light reaction) and thus restore...
Topics
- Amino Acid Sequence
- Binding Sites
- DNA Repair
- DNA, Bacterial
- DNA-Binding Proteins
- Deoxyribodipyrimidine Photo-Lyase
- Escherichia coli
- Lyases
- Molecular Sequence Data
- Mutation
- Photolysis
- Pyrimidine Dimers
- Quantum Theory
- Substrate Specificity
- Tryptophan