Article
A role for the protease-sensitive loop region of Shiga-like toxin 1 in the retrotranslocation of its A1 domain from the endoplasmic reticulum lumen.
The Journal of biological chemistry - 17 Jun 2005
LaPointe Paul, Wei Xin, Gariépy Jean
Abstract excerpt
Shiga-like toxin I (Slt-I) is a ribosome-inactivating protein that undergoes retrograde transport to the endoplasmic reticulum to exert its cytotoxic effect on eukaryotic cells. Its catalytically active A(1) domain subsequently migrates from the endoplasmic reticulum (ER) lumen to the cytoplasm. To study this final retrotranslocation event, a suicide assay was developed based on the cytoplasmic expression and...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Binding Sites
- Biochemistry
- Catalytic Domain
- Chlorocebus aethiops
- Circular Dichroism
- Cytoplasm
- Cytosol
- DNA Primers
- Dose-Response Relationship, Drug
- Endoplasmic Reticulum