Article
Identification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiae.
Toxicon : official journal of the International Society on Toxinology - 15 Mar 2011
Di Rong, Kyu Eric, Shete Varsha, Saidasan Hemalatha, Kahn Peter C, Tumer Nilgun E
Abstract excerpt
Shiga toxins (Stx1 and Stx2) are produced by E. coli O157:H7, which is a leading cause of foodborne illness. The A subunits of Stx1 (Stx1A) and Stx2 (Stx2A) are ribosome inactivating proteins (RIPs) that inhibit translation by removing an adenine from the highly conserved α-sarcin ricin loop (SRL) of the large rRNA. Here, we used mutagenesis in Saccharomyces cerevisiae to identify residues critical for...
Topics
- Amino Acids
- Apoptosis
- Image Processing, Computer-Assisted
- Mutation
- Protein Conformation
- Purines
- RNA, Ribosomal
- Ribosome Inactivating Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Shiga Toxin 1
- Shiga Toxin 2