Article
tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p.
RNA (New York, N.Y.) - 1 May 2005
Alexandrov Andrei, Grayhack Elizabeth J, Phizicky Eric M
Abstract excerpt
We show that Saccharomyces cerevisiae strains lacking Trm8p/Trm82p tRNA m7G methyltransferase are temperature-sensitive in synthetic media containing glycerol. Bacterial TRM8 orthologs complement the growth defect of trm8-Delta, trm82-Delta, and trm8-Delta trm82-Delta double mutants, suggesting that bacteria employ a single subunit for Trm8p/Trm82p function. The growth phenotype of trm8 mutants correlates with...
Topics
- Amino Acid Sequence
- Bacteria
- Catalysis
- Culture Media
- Genetic Complementation Test
- Glycerol
- Molecular Sequence Data
- Mutation
- Phenotype
- RNA Precursors
- RNA, Transfer, Phe
- RNA-Binding Proteins
- Saccharomyces cerevisiae
- Temperature