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Synthetic single-framework antibody library integrated with rapid affinity maturation by VL shuffling

16 Jun 2011

Abstract excerpt

Affinity maturation is often applied to improve the properties of antibodies isolated from universal antibody libraries in vitro. A synthetic human scFv antibody library was constructed in single immunoglobulin framework to enable rapid affinity maturation by updated Kunkel's mutagenesis. The initial diversity was generated predominantly in the V(H) domain combined with only 36 V(L) domain variants yielding 3 ×...

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Share to Glycosylation and Glycoproteins Research
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PMID: 21680620
DOI: 10.1093/protein/gzr023

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