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Article

Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display.

Nature biotechnology - 1 Dec 2000

Hanes J, Schaffitzel C, Knappik A, Plückthun A

Abstract excerpt

Here we applied ribosome display to in vitro selection and evolution of single-chain antibody fragments (scFvs) from a large synthetic library (Human Combinatorial Antibody Library; HuCAL) against bovine insulin. In three independent ribosome display experiments different clusters of closely related scFvs were selected, all of which bound the antigen with high affinity and specificity. All selected scFvs had...

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Identifiers

PMID: 11101809
DOI: 10.1038/82407

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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