Article
High throughput mutational characterization of the GPCR ligand C5a using yeast display and deep sequencing.
Structure (London, England : 1993) - 8 Jan 2026
Xu Yu, Thakkar Kaushik, Guan Li, Miao Yu, Mehibel Manal, Lee Robert B, Marciano David, Viswanathan Vignesh, Wang Ziwei, Wang Jinglong, Ji Lu, Cao Hongbin, Petrakian Camille Fisher, Valenzuela Jocelyn, LaGory Edward, Jia Xianglian, Moon Eui Jung, Martinez Rodolph, Wu Fang, Frock Richard L, Moding Everett J, Le Quynh-Thu, Rankin Erinn B, Zhang Cheng, Huang Possu, Olcina Monica M, Giaccia Amato J, Graves Edward E
Abstract excerpt
High-throughput mutagenesis approaches are widely employed to systematically characterize protein functions and play a critical role in therapeutic developments. As the largest class of membrane receptors, G protein-coupled receptors (GPCRs) are a primary focus of these studies. However, while significant progress has been made in understanding GPCRs themselves, mutagenesis studies on their ligands have lagged...
Topics
- Receptor, Anaphylatoxin C5a
- Humans
- Ligands
- Protein Binding
- Complement C5a
- Mutation
- High-Throughput Nucleotide Sequencing
- Binding Sites