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Article

Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451.

Nucleic acids research - 1 Jan 2005

Erlacher Matthias D, Lang Kathrin, Shankaran Nisha, Wotzel Brigitte, Hüttenhofer Alexander, Micura Ronald, Mankin Alexander S, Polacek Norbert

Abstract excerpt

The main enzymatic reaction of the large ribosomal subunit is peptide bond formation. Ribosome crystallography showed that A2451 of 23S rRNA makes the closest approach to the attacking amino group of aminoacyl-tRNA. Mutations of A2451 had relatively small effects on transpeptidation and failed to unequivocally identify the crucial functional group(s). Here, we employed an in vitro reconstitution system for...

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Identifiers

PMID: 15767286
DOI: 10.1093/nar/gki308
PMCID: PMC1065261

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Source receipt: pubmed, retrieved 7 Sept 2026.

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