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Article

A single adenosine with a neutral pKa in the ribosomal peptidyl transferase center.

Science (New York, N.Y.) - 11 Aug 2000

Muth G W, Ortoleva-Donnelly L, Strobel S A

Abstract excerpt

Biochemical and crystallographic evidence suggests that 23S ribosomal RNA (rRNA) is the catalyst of peptide bond formation. To explore the mechanism of this reaction, we screened for nucleotides in Escherichia coli 23S rRNA that may have a perturbed pKa (where Ka is the acid constant) based on the pH dependence of dimethylsulfate modification. A single universally conserved A (number 2451) within the central loop...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 10937997
DOI: 10.1126/science.289.5481.947

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A single adenosine with a neutral pKa in the ribosomal peptidyl transferase center.PMID 10937997Read ↗

The A2453-C2499 wobble base pair in Escherichia coli 23S ribosomal RNA is responsible for pH sensitivity of the peptidyltransferase active site conformation.Semantic proximity Read ↗

Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide.Semantic proximity Read ↗

Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451.Semantic proximity Read ↗

Important contribution to catalysis of peptide bond formation by a single ionizing group within the ribosome.Semantic proximity Read ↗

The G2447A mutation does not affect ionization of a ribosomal group taking part in peptide bond formation.Semantic proximity Read ↗

The interaction between C75 of tRNA and the A loop of the ribosome stimulates peptidyl transferase activity.Semantic proximity Read ↗

Mutations in the conserved P loop perturb the conformation of two structural elements in the peptidyl transferase center of 23 S ribosomal RNA.Semantic proximity Read ↗

Mutagenesis of the peptidyltransferase center of 23S rRNA: the invariant U2449 is dispensable.Semantic proximity Read ↗

Mutational characterization and mapping of the 70S ribosome active site.Semantic proximity Read ↗

The Positively Charged Active Site of the Bacterial Toxin RelE Causes a Large Shift in the General Base pKa.Semantic proximity Read ↗

Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit.Semantic proximity Read ↗

Bases defining an ammonium and magnesium ion-dependent tertiary structure within the large subunit ribosomal RNA.Semantic proximity Read ↗

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