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Article

Selective fluorescence recovery after bleaching of single E2GFP proteins induced by two-photon excitation.

Chemphyschem : a European journal of chemical physics and physical chemistry - 1 Feb 2005

Chirico G, Diaspro A, Cannone F, Collini M, Bologna S, Pellegrini V, Beltram F

Abstract excerpt

We report the two-photon excitation and emission or a recently developed green fluorescent protein (GFP) mutant, E(2)GFP. Two main excitation bands are found at 780 and 870 nm. Blinking and irreversible and reversible bleaching were observed. Fluorescence blinking occurs in the millisecond range and has been ascribed to conversions between the neutral, anionic and dark zwitterionic states. Bleaching is observed...

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PMID: 15751356
DOI: 10.1002/cphc.200400318

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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