Article
Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET.
Chemistry & biology - 30 Jul 2010
Subach Fedor V, Zhang Lijuan, Gadella Theodorus W J, Gurskaya Nadya G, Lukyanov Konstantin A, Verkhusha Vladislav V
Abstract excerpt
We have developed the first red fluorescent protein, named rsTagRFP, which possesses reversibly photoswitchable absorbance spectra. Illumination with blue and yellow light switches rsTagRFP into a red fluorescent state (ON state) or nonfluorescent state (OFF state), respectively. The ON and OFF states exhibit absorbance maxima at 567 and 440 nm, respectively. Due to the photoswitchable absorbance, rsTagRFP can be...
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