Article
Semi-rational engineering of a coral fluorescent protein into an efficient highlighter.
EMBO reports - 1 Mar 2005
Tsutsui Hidekazu, Karasawa Satoshi, Shimizu Hideaki, Nukina Nobuyuki, Miyawaki Atsushi
Abstract excerpt
Kaede is a natural photoconvertible fluorescent protein found in the coral Trachyphyllia geoffroyi. It contains a tripeptide, His 62-Tyr 63-Gly 64, which acts as a green chromophore that is photoconvertible to red following (ultra-) violet irradiation. Here, we report the molecular cloning and crystal structure determination of a new fluorescent protein, KikG, from the coral Favia favus, and its in vitro...
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