Disulfide mutants of barnase. I: Changes in stability and structure assessed by biophysical methods and X-ray crystallography.

Abstract excerpt

In this series of papers, we examine the effects of introducing disulfide bonds on the properties, structure and thermodynamics of a small globular protein, barnase. Three mutants have been made, in each of which a single crosslink confers different properties. Two of the disulfide bonds, between...

Topics

• Bacillus
• Bacterial Proteins
• Biophysical Phenomena
• Biophysics
• Crystallography, X-Ray
• Disulfides
• Enzyme Stability
• Models, Molecular
• Molecular Structure
• Mutation
• Oxidation-Reduction
• Ribonucleases
• Thermodynamics