Article
A structural double-mutant cycle: estimating the strength of a buried salt bridge in barnase.
Acta crystallographica. Section D, Biological crystallography - 1 Apr 2002
Vaughan Cara K, Harryson Pia, Buckle Ashley M, Fersht Alan R
Abstract excerpt
Double-mutant cycles are widely used in the field of protein engineering to measure intermolecular and intramolecular interactions. Ideally, there should be no structural rearrangement of the protein on making the two single mutations and the double mutation within the cycle. However, structural pertubation on mutation does not preclude the use of this method, providing the sum of the changes in the single...
Topics
- Arginine
- Bacterial Proteins
- Models, Molecular
- Mutation
- Ribonucleases
- Thermodynamics