Article
Incorporation of dA opposite N3-ethylthymidine terminates in vitro DNA synthesis.
Biochemistry - 13 Nov 1990
Bhanot O S, Grevatt P C, Donahue J M, Gabrielides C N, Solomon J J
Abstract excerpt
N3-Ethylthymidine (N3-Et-dT) was site specifically incorporated into a 17-nucleotide oligomer to investigate the significance of DNA ethylation at the central hydrogen-bonding site (N3) of thymine. The 5'-(dimethoxytrityl)-protected N3-Et-dT was converted to the corresponding 3'-phosphoramidite and used to incorporate N3-Et-dT at a single site in the oligonucleotide during synthesis by the phosphite triester...
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