Incorporation of dA opposite N3-ethylthymidine terminates in vitro DNA synthesis.

doi:10.1021/bi00497a010

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Article

Incorporation of dA opposite N3-ethylthymidine terminates in vitro DNA synthesis.

Biochemistry - 13 Nov 1990

Bhanot O S, Grevatt P C, Donahue J M, Gabrielides C N, Solomon J J

Abstract excerpt

N3-Ethylthymidine (N3-Et-dT) was site specifically incorporated into a 17-nucleotide oligomer to investigate the significance of DNA ethylation at the central hydrogen-bonding site (N3) of thymine. The 5'-(dimethoxytrityl)-protected N3-Et-dT was converted to the corresponding 3'-phosphoramidite a...

Topics

Bacteriophage phi X 174
Base Composition
Base Sequence
DNA
Deoxyadenine Nucleotides
Ethylnitrosourea
Hydrogen Bonding
Molecular Sequence Data
Mutation
Templates, Genetic
Thymidine

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