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Article

Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity.

Chembiochem : a European journal of chemical biology - 2 Jan 2007

Reetz Manfred T, Puls Michael, Carballeira José Daniel, Vogel Andreas, Jaeger Karl-Erich, Eggert Thorsten, Thiel Walter, Bocola Marco, Otte Nikolaj

Abstract excerpt

An earlier experimental study, which involved the directed evolution of enantioselective lipase variants from Pseudomonas aeruginosa as catalysts in the hydrolytic kinetic resolution of 2-methyl-decanoic acid p-nitrophenyl ester, provided a mutant with six mutations. Consequently, the selectivity factor was found to increase from E = 1.1 for the wild-type to E = 51 for the best mutant. Only one of the amino acid...

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PMID: 17133645
DOI: 10.1002/cbic.200600359

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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