Article
Selection and characterization of a promoter for expression of single-copy recombinant genes in Gram-positive bacteria.
BMC biotechnology - 14 Jan 2005
Provvedi Roberta, Maggi Tiziana, Oggioni Marco R, Manganelli Riccardo, Pozzi Gianni
Abstract excerpt
BACKGROUND: In the past ten years there has been a growing interest in engineering Gram-positive bacteria for biotechnological applications, including vaccine delivery and production of recombinant proteins. Usually, bacteria are manipulated using plasmid expression vectors. The major limitation of this approach is due to the fact that recombinant plasmids are often lost from the bacterial culture upon removal of...
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