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Article

Genetic selection system for improving recombinant membrane protein expression in E. coli.

Protein science : a publication of the Protein Society - 1 Feb 2009

Massey-Gendel Elizabeth, Zhao Anni, Boulting Gabriella, Kim Hye-Yeon, Balamotis Michael A, Seligman Len M, Nakamoto Robert K, Bowie James U

Abstract excerpt

A major barrier to the physical characterization and structure determination of membrane proteins is low yield in recombinant expression. To address this problem, we have designed a selection strategy to isolate mutant strains of Escherichia coli that improve the expression of a targeted membrane protein. In this method, the coding sequence of the membrane protein of interest is fused to a C-terminal selectable...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 19165721
DOI: 10.1002/pro.39
PMCID: PMC2708063

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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