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Construction and Characterization of a Lactose-Inducible Promoter System for Controlled Gene Expression in <i>Clostridium perfringens</i>

20 Nov 2010

Abstract excerpt

Clostridium perfringens is a Gram-positive anaerobic pathogen which causes many diseases in humans and animals. While some genetic tools exist for working with C. perfringens, a tightly regulated, inducible promoter system is currently lacking. Therefore, we constructed a plasmid-based promoter system that provided regulated expression when lactose was added. This plasmid (pKRAH1) is an Escherichia coli-C....

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PMID: 21097603
DOI: 10.1128/aem.01536-10

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