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Article

Studies on the genomic organization of recombinant Streptococcus gordonii and the development of a novel intergenic integration site for foreign gene expression.

Journal of molecular microbiology and biotechnology - 1 Oct 2001

Franke C A, Bolken T C, Hruby D E

Abstract excerpt

The methods currently employed to produce recombinant Streptococcus gordonii strains for use as vaccines and/or protein expression vectors result in the insertion of foreign genes into an unknown integration site with no information on the transcriptional context or potential phenotypic consequences. Therefore, the genomic organization surrounding the insertion site of a recombinant strain of S. gordonii (GP1223)...

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PMID: 11545273

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