Article
Construction and application of an expression vector from the new plasmid pLAtc1 of Acidithiobacillus caldus.
Applied microbiology and biotechnology - 1 May 2014
Zhang Ming-Jiang, Jiang Cheng-Ying, You Xiao-Yan, Liu Shuang-Jiang
Abstract excerpt
In this study, a recently sequenced 9.8-kb plasmid, pLAtc1, from Acidithiobacillus caldus strain SM-1 was characterized and developed into an expression vector. The pLAtc1 backbone carried an oriV, three rep genes, five mob genes, a Nic site, and an addiction system. Multilocus sequence analysis indicated that pLAtc1 was phylogenetically more related to the IncQ-like broad host range plasmids than to other IncQ...
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