Article
A new simple diagnostic assay for the identification of the major CYP2D6 genotypes by DNA sequencing analysis.
International journal of clinical pharmacology and therapeutics - 1 Dec 2004
James H M, Coller J K, Gillis D, Bahnisch J, Sallustio B C, Somogyi A A
Abstract excerpt
AIM: To establish a method suitable for diagnostic genotyping of CYP2D6 alleles by DNA sequencing. METHODS: Initial PCR reactions were performed to specifically amplify exons 3, 4, 5 and 6 of the CYP2D6 gene using primers previously published. New primers were used to identify *2, *3, *4, *6, *7, *8, *9 and *41 in 2 sequencing reactions. Additional primers were designed for reverse sequencing in samples with 1 or...
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