Functionalization of tumor necrosis factor-alpha using phage display technique and PEGylation improves its antitumor therapeutic window.

doi:10.1158/1078-0432.CCR-04-0770

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Article

Functionalization of tumor necrosis factor-alpha using phage display technique and PEGylation improves its antitumor therapeutic window.

Clinical cancer research : an official journal of the American Association for Cancer Research - 15 Dec 2004

Shibata Hiroko, Yoshioka Yasuo, Ikemizu Shinji, Kobayashi Kyoko, Yamamoto Yoko, Mukai Yohei, Okamoto Takayuki, Taniai Madoka, Kawamura Maki, Abe Yasuhiro, Nakagawa Shinsaku, Hayakawa Takao, Nagata Satoshi, Yamagata Yuriko, Mayumi Tadanori, Kamada Haruhiko, Tsutsumi Yasuo

Abstract excerpt

PURPOSE: In this study, the optimization of antitumor therapy with tumor necrosis factor-alpha (TNF-alpha) was attempted. EXPERIMENTAL DESIGN: Using the phage display technique, we created a lysine-deficient mutant TNF-alpha (mTNF-K90R). This mutant had higher affinities to both TNF receptors, despite reports that certain lysine residues play important roles in trimer formation and receptor binding. RESULTS: The...

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PMID: 15623605
DOI: 10.1158/1078-0432.CCR-04-0770

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