Article
[Establishment of a new HBV genotyping method with PCR-RBD and its application].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Nov 2004
Yang Guang, Cui Jin-huan, Chen Shu, Si Jian-hua, Tan Jia-ju, Li Pei-yuan, Ma Xin
Abstract excerpt
OBJECTIVE: Using PCR-RDB to establish a new method for HBV genotyping, and to survey the distribution of HBV genotypes in the Foshan area. METHODS: Biotin-labeled primers for amplification of HBV region X (nt1550-1789) were used to amplify extracted HBV DNA. HBV was genotyped by hybridization of the PCR products with immobilized specific probes (genotype A to F) on C membrane. Color development was achieved by...
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