Article
[A novel method for hepatitis C virus genotyping using RT-PCR reverse dot blot hybridization technique].
Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 1 Oct 2010
Wei Jun-feng, Zhang Tai-song, Huang Hui-hong, Zeng Yan-li, Zhang Fan, Wang Jun-jie, Zhou Bin, Wu Ying-song, Liu Shu-wen, Hou Jin-lin, Guo Ya-bing, Zhou Yuan-ping
Abstract excerpt
OBJECTIVE: To develop a rapid and specific method for hepatitis C virus ( HCV) genotyping using reverse dot blot hybridization technique and investigate the distribution of HCV genotypes and subtypes in Guangdong. METHODS: The primers and the probes targeting the 5'untranslated region (5'UTR) and core region of HCV genotypes 1b, 2a, 3a, 3b and 6a were designed, and the RT-PCR reverse dot blot hybridization...
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