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Article

[Development and application of a new hepatitis C virus genotyping method with polymerase chain reaction-reverse blot dot technique].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Jun 2005

Yang Guang, Chen Shu, Cui Jin-huan, Si Jian-hua, Tan Jia-ju

Abstract excerpt

OBJECTIVE: Using polymerase chain reaction-reverse blot dot (PCR-RDB) technique to establish a new method for hepatitis C virus (HCV) genotyping and to study the distribution of HCV genotypes in Foshan area. METHODS: HCV primers and probes were designed in 5'-untranslated region (nt-1-nt-299) of HCV. HCV RNA in serum was isolated and purified, and its cDNA was obtained by reversed transcription. Nested PCR using...

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PMID: 16185461

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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