Article
[Establishment and application of real-time fluoriescene-based metry polymerase chain reaction for hepatitis B virus genotyping].
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA - 1 Jun 2005
Shen Jian-kun, Zhou Rong, Wang Zhan-hui, Bai Pei-sheng, Ma Shi-wu, Zhang Ke, Hou Jin-lin
Abstract excerpt
OBJECTIVE: To explore a new method for determining hepatitis B virus (HBV) genotypes B-D with real-time fluorescence-based PCR. METHODS: The PCR primers and probes were designed according to the analysis of 143 complete HBV genomes from GenBank and on the basis of a search for genotype-specific nucleotide sequences which were conserved in the 3 HBV genotypes. Real-time fluorescence-based PCR was performed for HBV...
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