Article
[Establishment of a nested PCR to identify hepatitis B virus genotypes A-D and subgenotypes B1, B2, C1 and C2].
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi - 1 Dec 2008
Jin Hui, Wang Jie, Yan Ling, Nie Jing-jing, Li Jie, Zhuang Hui
Abstract excerpt
OBJECTIVE: To establish a hepatitis B virus (HBV) nested PCR (nPCR) for detection of genotypes A-D and subgenotypes B1, B2, C1 and C2. METHODS: The entire HBV nucleotide sequences of genotypes A-H retrieved from GenBank were compared and analyzed by DNAStar software. The PCR primers were designed by Primer Premier 5.0 software, and the nPCR for genotyping HBV/A-D as well as subgenotyping B1, B2, C1 and C2 were...
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