Antibody framework residues affecting the conformation of the hypervariable loops.

doi:10.1016/0022-2836(92)91010-m

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Article

Antibody framework residues affecting the conformation of the hypervariable loops.

Journal of molecular biology - 20 Mar 1992

Foote J, Winter G

Abstract excerpt

Rodent monoclonal antibodies have been "humanized" or "reshaped" for therapy by transplanting the antigen-binding loops from their variable domains onto the beta-sheet framework regions of human antibodies. However, additional substitutions in the human framework regions are sometimes required for high affinity antigen binding. Here we describe antigen binding by a reshaped antibody derived from the mouse...

Topics

Amino Acid Sequence
Animals
Antibodies, Monoclonal
Base Sequence
Binding Sites, Antibody
Crystallography
DNA
Humans
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mice

Models, Molecular

Molecular Sequence Data

Muramidase

Mutation

Protein Conformation

Spectrometry, Fluorescence

Source

Open in PubMed

Identifiers

PMID: 1560463
DOI: 10.1016/0022-2836(92)91010-m

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1560463-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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