Article
Structural consequences of humanizing an antibody.
Journal of immunology (Baltimore, Md. : 1950) - 1 Mar 1997
Holmes M A, Foote J
Abstract excerpt
We have determined the crystal structure of the Fv fragment of a humanized anti-hen eggwhite lysozyme Ab (HuLys). This molecule is a composite of known structures: complementarity-determining regions (CDRs) were from the mouse anti-lysozyme Ab D1.3, heavy chain framework regions were from the hum...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites, Antibody
- Crystallography, X-Ray
- Humans
- Immunoglobulin Fragments
- Immunoglobulin Variable Region
- Mice
- Molecular Sequence Data
- Muramidase
- Mutation
- Protein Conformation
- Protein Engineering
- Structure-Activity Relationship