Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Effect of oligonucleotide primers in determining viral variability within hosts.

Virology journal - 9 Dec 2004

Bracho Maria Alma, García-Robles Inmaculada, Jiménez Nuria, Torres-Puente Manuela, Moya Andrés, González-Candelas Fernando

Abstract excerpt

BACKGROUND: Genetic variability in viral populations is usually estimated by means of polymerase chain reaction (PCR) based methods in which the relative abundance of each amplicon is assumed to be proportional to the frequency of the corresponding template in the initial sample. Although bias in template-to-product ratios has been described before, its relevance in describing viral genetic variability at the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Primers
Share to Hepacivirus
Share to Hepatitis C

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Oligonucleotides

Share to Phylogeny

Share to Reproducibility of Results

Share to RNA, Viral

Share to Sensitivity and Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 15588294
DOI: 10.1186/1743-422X-1-13
PMCID: PMC543450

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Effect of oligonucleotide primers in determining viral variability within hosts.PMID 15588294Read ↗

Sampling and repeatability in the evaluation of hepatitis C virus genetic variability.Semantic proximity Read ↗

Do Taq-generated RT-PCR products from RNA viruses accurately reflect viral genetic heterogeneity?Semantic proximity Read ↗

Experimental analysis of sources of error in evolutionary studies based on Roche/454 pyrosequencing of viral genomes.Semantic proximity Read ↗

Inference with viral quasispecies diversity indices: clonal and NGS approaches.Semantic proximity Read ↗

Diversity of hepatitis G virus within a single infected individual.Semantic proximity Read ↗

Inferred hepatitis C virus quasispecies diversity is influenced by choice of DNA polymerase in reverse transcriptase-polymerase chain reactions.Semantic proximity Read ↗

Design of primers for PCR ampiification of highly variable genomesSemantic proximity Read ↗

Towards prediction of HCV therapy efficiency.Semantic proximity Read ↗

The impact of PCR-generated recombination on diversity estimation of mixed viral populations by deep sequencing.Semantic proximity Read ↗

From RNA to quasispecies: a DNA polymerase with proofreading activity is highly recommended for accurate assessment of viral diversity.Semantic proximity Read ↗

Diversity of hepatitis C virus quasispecies evaluated by denaturing gradient gel electrophoresis.Semantic proximity Read ↗

Variability of the polymerase gene (NS5B) in hepatitis C virus-infected women.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.