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Article

The impact of PCR-generated recombination on diversity estimation of mixed viral populations by deep sequencing.

Journal of virological methods - 1 Oct 2010

Görzer Irene, Guelly Christian, Trajanoski Slave, Puchhammer-Stöckl Elisabeth

Abstract excerpt

Ultra-deep pyrosequencing (UDPS) of targeted amplicons allows to determine a large number of individual sequence reads from a single PCR product, and this approach is thus extremely valuable for analysis of mixed viral populations. A mixture of genetically distinct DNA templates, however, may lead to the generation of recombinant sequences during the initial PCR amplification step. In the present study, the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 20691210
DOI: 10.1016/j.jviromet.2010.07.040

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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