Article
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
Proceedings of the National Academy of Sciences of the United States of America - 15 Mar 1994
Barnes W M
Abstract excerpt
A target length limitation to PCR amplification of DNA has been identified and addressed. Concomitantly, the base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased. These improvements were achieved by the combination of a high level...
Topics
- Bacteriophage lambda
- Base Composition
- Base Sequence
- DNA Primers
- DNA, Viral
- Exonucleases
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutation
- Particle Size
- Polymerase Chain Reaction
- Templates, Genetic