Article
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
Proceedings of the National Academy of Sciences of the United States of America - 15 Mar 1994
Barnes W M
Abstract excerpt
A target length limitation to PCR amplification of DNA has been identified and addressed. Concomitantly, the base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased. These improvements were achieved by the combination of a high level of an exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase, Klentaq1, with a very low level of a...
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