Article
Relocation of a cytoplasmic yeast linear plasmid to the nucleus is associated with circularization via nonhomologous recombination involving inverted terminal repeats.
Molecular & general genetics : MGG - 1 Jun 2000
Gunge N, Takata H, Fukuda K, Iwao S, Miyakawa I
Abstract excerpt
The linear plasmid pCLU1 from the yeast Kluyveromyces lactis normally replicates in the cytoplasm, with the aid of the helper linear plasmid pGKL2, using terminal protein (TP) as a primer. However, it relocates to the nucleus when selection is applied for the expression of a plasmid-borne nuclear marker. Migration to the nucleus occurred in K. lactis at a frequency of about 10(-3)/cell ten or more times higher...
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