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Article

Purification and characterization of the<i>in vitro</i>activity of I-<i>Sce</i>I, a novel and highly specific endonuclease encoded by a group I intron

1 Jan 1990

Abstract excerpt

Group I intron encoded proteins represent a novel class of site specific double strand endonucleases. The endonuclease activity of this class of proteins has been first demonstrated in vivo for I-Sce I which is encoded by a mitochondrial intron of Saccharomyces cerevisiae. Assays using crude cell extracts have shown that I-Sce I can be used in vitro as a restriction endonuclease potentially useful for recombinant...

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PMID: 2183191
DOI: 10.1093/nar/18.6.1407

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